Figure 5. T-lymphocytes control the severity of innate inflammatory responses.

(A) Cytokine production in vitro by male and female resident peritoneal cells (2x10⁵ cells/sample, n=6 mice) following 3h stimulation by TLR4-specific LPS (0.1µg/ml) or TLR2 agonist Pam3CSK4 (Pam3, 0.1µg/ml). (B) TNFα production by isolated resident male peritoneal macrophage (1.5x10⁵ cells/sample, n=3 mice) treated with LPS (0.1µg/ml, 18h) in the absence or presence of CD4+ve T lymphocytes (1.5x10⁵ cells). (C) Zymosan-induced (1mg, ip, 3h) recruitment of GR1+ granulocytes into peritoneal cavity of C57BL/6 (wild type) and T-lymphocyte deficient Rag2 knockout (KO) mice (n=5 mice). All values are expressed as mean ± sem. #P<0.05 by one-way ANOVA compared to male and § P<0.05 by one-way ANOVA relative to macrophage alone. *P<0.05 by Students t-test relative to wild type.