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The cell lysates were analyzed via Western blotting for p-Akt and t-Akt. (A) representative image; Akt phosphorylation was determined from C2C12 cells, and treated with 40 μg/ mL of BB for the indicated period of time (5–60 min); (B). The ratios of phospho-Akt to total Akt forms were analyzed and presented phosphorylation of Akt.
