Table 1. Primers used in this study.
Gene | Accession number | Forward primer and reverse primer | PCR product (bp) | Annealing temperature (°C) |
---|---|---|---|---|
Liver | ||||
PEPCK | NM_011044.2 | F: CTACAACTTCGGCAAATACC | 330 | 52 |
R: TCCAGATACCTGTCGATCTC | ||||
G6Pase | NM_008061.3 | F: GAACAACTAAAGCCTCTGAAAC | 350 | 50 |
R: TTGCTCGATACATAAAACACTC | ||||
11β-HSD1 | NM_008288.2 | F:AAGCAGAGCAATGGCAGCAT | 300 | 50 |
R: GAGCAATCATAGGCTGGGTCA | ||||
PPARα | NM_011144 | F: ACCTCTGTTCATGTCAGACC | 352 | 55 |
R: ATAACCACAGACCAACCAAG | ||||
SREBP1c | NM_011480 | F: GGCTGTTGTCTACCATAAGC | 219 | 50 |
R: AGGAAGAAACGTGTCAAGAA | ||||
CPT1a | BC054791.1 | F:CTTGTGACCCTACTACATCC | 332 | 51 |
R:TCATAGCAGAACCTTAATCC | ||||
aP2 | NM_024406 | F:TCACCTGGAAGACAGCTCCT | 142 | 52 |
R:TGCCTGCCACTTTCCTTGT | ||||
SREBP2 | AF289715.2 | F: ATATCATTGAAAAGCGCTAC | 256 | 48 |
R: ATTTTCAAGTCCACATCACT | ||||
GAPDH | NM_031144 | F: TGTGTCCGTCGTGGATCTGA | 99 | 55 |
R: CCTGCTTCACCACCTTCTTGA |