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. 2017 Mar 23;12(3):e0173984. doi: 10.1371/journal.pone.0173984

Table 1. Primers used in this study.

Gene Accession number Forward primer and reverse primer PCR product (bp) Annealing temperature (°C)
Liver
PEPCK NM_011044.2 F: CTACAACTTCGGCAAATACC 330 52
R: TCCAGATACCTGTCGATCTC
G6Pase NM_008061.3 F: GAACAACTAAAGCCTCTGAAAC 350 50
R: TTGCTCGATACATAAAACACTC
11β-HSD1 NM_008288.2 F:AAGCAGAGCAATGGCAGCAT 300 50
R: GAGCAATCATAGGCTGGGTCA
PPARα NM_011144 F: ACCTCTGTTCATGTCAGACC 352 55
R: ATAACCACAGACCAACCAAG
SREBP1c NM_011480 F: GGCTGTTGTCTACCATAAGC 219 50
R: AGGAAGAAACGTGTCAAGAA
CPT1a BC054791.1 F:CTTGTGACCCTACTACATCC 332 51
R:TCATAGCAGAACCTTAATCC
aP2 NM_024406 F:TCACCTGGAAGACAGCTCCT 142 52
R:TGCCTGCCACTTTCCTTGT
SREBP2 AF289715.2 F: ATATCATTGAAAAGCGCTAC 256 48
R: ATTTTCAAGTCCACATCACT
GAPDH NM_031144 F: TGTGTCCGTCGTGGATCTGA 99 55
R: CCTGCTTCACCACCTTCTTGA