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. 2017 Mar 2;50(4):1392–1402. doi: 10.3892/ijo.2017.3894

Figure 4.

Figure 4

Effect of 3-HT on DNA damage and cell cycle regulatory proteins in A2780/CP70 and OVCAR-3 cells. (A) The DNA damage regulatory proteins in A2780/CP70 and OVCAR-3 cells were detected by western blotting, cells were incubated with 3-HT at 0–8 µM for 24 h, cell lysates were prepared and then subjected to western blotting, GAPDH was used as internal control. (B and C) A2780/CP70 and OVCAR-3 protein expression data were expressed as means ± SEM of three independent experiments. *P<0.05, **P<0.01, ***P<0.001. (D) The cell cycle regulatory proteins in A2780/CP70 and OVCAR-3 cells were detected by western blotting, cells were incubated with 3-HT at 0–8 µM for 24 h, cell lysates were prepared and then subjected to western blotting, GAPDH was used as internal control. (E and F) A2780/CP70 and OVCAR-3 protein expression data were expressed as means ± SEM of three independent experiments. *P<0.05, **P<0.01, ***P<0.001.