Figure 5.

3-HT induces ROS generation and activates the MAPK pathway. (A and B) ROS generation in A2780/CP70 and OVCAR-3 cells were detected using DCFH-DA. Cells were treated with 3-HT for 24 h, then incubated with DCFH-DA, fluorescence intensity was measured by fluorescence microplate reader. Data were expressed as mean ± SEM of three independent experiments. ^*P<0.05, ^**P<0.01. (C) Protein levels of MAPK family in A2780/CP70 and OVCAR-3 cells were analysed by western blotting. Cells were treated with 3-HT for 24 h, cell lysates were then prepared and subjected to western blotting to detect the protein levels, GAPDH was used as internal control. (D and E) A2780/CP70 and OVCAR-3 protein expression data were expressed as means ± SEM of three independent experiments. ^*P<0.05, ^**P<0.01, ^***P<0.001.