Figure 6.

Effect of 3-HT on the intrinsic and extrinsic apoptotic pathways in A2780/CP70 and OVCAR-3 cells. (A) The intrinsic apoptotic related proteins were detected by western blotting. The cells were treated with 3-HT for 24 h. Cell lysates were prepared and then subjected to western blotting to detect the protein levels. GAPDH was used as internal control. (B and C) A2780 and OVCAR-3 protein expression data were expressed as means ± SEM of three independent experiments. ^*P<0.05, ^**P<0.01, ^***P<0.001. (D) The intrinsic apoptotic related proteins were detected by western blotting. The cells were treated with 3-HT for 24 h. Cell lysates were prepared and then subjected to western blotting to detect the protein levels. GAPDH was used as internal control. (E and F) A2780/CP70 and OVCAR-3 protein expression data were expressed as means ± SEM of three independent experiments. ^*P<0.05, ^**P<0.01, ^***P<0.001.