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. 2017 Feb 14;50(4):1109–1115. doi: 10.3892/ijo.2017.3876

Figure 3.

Figure 3

Effects of miR-21 on the expression of KLF4, E-cadherin, N-cadherin, vimentin and/or Akt/ERK1/2 pathways. (A) The relative levels of p-Akt, Akt, p-ERK, ERK, KLF4 and EMT marker proteins were measured by western blot analysis. (B-D) Bands were semi-quantified using Quantity One software. (E) Western blot analysis showing protein levels of p-Akt, Akt, p-ERK1/2, ERK1/2, KLF4, mesenchymal markers (N-cadherin and vimentin) and epithelial cell marker (E-cadherin). (F-I) Bands were semi-quantified using Quantity One software. GAPDH was used as loading control (**P<0.01).