Skip to main content
. 2017 Mar 10;50(4):1097–1108. doi: 10.3892/ijo.2017.3909

Figure 2.

Figure 2

miR-150 inhibits the proliferation of nasopharyngeal carcinoma cells in vitro. (A) Downregulation of miR-150 positively correlated with clinical stages of nasopharyngeal carcinoma. (B) miR-150 expression was detected by real-time PCR analysis. miR-150 expression was examined in overexpressed miR-150 and anti-miR-150 NPC cells, and their respective control vectors. U6 was used as the loading control. Each bar represents the mean values ± SD of three independent experiments. *P<0.05. (C) CCK-8 assay revealed that overexpression of miR-150 decreased, while downregulation of endogenous miR-150 increased the proliferation rate in CNE-2 and HONE1 cells. Each bar represents the mean values ± SD of three independent experiments. (D) Colony formation assay revealed that overexpression of miR-150 decreased, while downregulation of endogenous miR-150 increased the colony-forming ability in CNE-2 and HONE1 cells. Each bar represents the mean values ± SD of three independent experiments. *P<0.05. (E) Anchorage-independent growth assays revealed that overexpression of miR-150 decreased, while downregulation of endogenous miR-150 increased the anchorage-independent growth ability in CNE-2 and HONE1 cells. Each bar represents the mean values ± SD of three independent experiments. *P<0.05.