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. 2017 Mar 15;50(4):1064–1074. doi: 10.3892/ijo.2017.3914

Figure 3.

Figure 3

Treatment effects on DNA damage. Three HGSOC cell lines were treated as described then assessed by staining for γ-H2AX and confocal immunofluorescence imaging. DMSO (0.002%) treatment was used as a vehicle control. (A) Representative images of at least four independent experiments are shown. γ-H2AX foci are colored in magenta, and nuclei are colored blue. Scale bar, 10 µm. (B) The percentage of γ-H2AX foci-positive cells (>5 γ-H2AX foci in nucleus) was determined by analyzing >120 cells per condition and shown as the mean ± SD of at least four independent experiments. P-values were calculated by a two-way ANOVA.