Figure 6.

UBE2C deficiency inhibits the expression of p-AURKA. (A) Protein levels of AURKA and p-AURKA were evaluated by western blot analysis in the si-UBE2C-treated gastric cell lines. (B) mRNA levels of AURKA expression in the indicated cell lines evaluated by quantitative PCR. (C) A large number of AURKA and UBE2C cells were observed in the cytoplasm; the level of AURKA was increased in si-UBE2C-treated cells. (D) Conversely, UBE2C expression was decreased slightly in Lenti-si-AURKA tumors in nude mice. UBE2C, ubiquitin-conjugating enzyme 2C; p-AURKA, phosphorylation AURKA; AURKA, Aurora-A.