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. 2017 Mar 23;12(3):e0174122. doi: 10.1371/journal.pone.0174122

Fig 6. Transcriptional regulation of Jag2 by TAp63γ.

Fig 6

(A) Region around exon 2 of the mouse Jag2 gene. Four consensus sequences for p63 binding in this region are shown as C1–4. (B) Luciferase activities in B16 melanoma cells co-transfected with a luciferase reporter gene construct containing a fragment (+423 bp to +2,309 bp) of the Jag2 gene ligated to a miniP and an expression vector for GFP, ΔNp63γ, or TAp63γ. RLA, relative luciferase activity. Error bars indicate s.d. (n = 3 biological replicates). **P < 0.01 (unpaired two-tailed Student's t test). (C) Luciferase activities in B16 melanoma cells co-transfected with luciferase reporter gene constructs containing the indicated fragments ligated to a miniP and an expression vector for GFP or TAp63γ. Error bars indicate s.d. (n = 3 biological replicates). *P < 0.05, **P < 0.01 vs. GFP. #P < 0.05, ##P < 0.01 vs. WT with TAp63γ (unpaired two-tailed Student's t test). (D) ChIP-qPCR using lysates of mouse ES cells transfected with 3×FLAG-tagged TAp63γ. An amplicon of each primer set is indicated as Q1–7 in the scheme. The negative control primers were designed in the upstream region of Aldh1a2 (Cntl). Error bars indicate s.d. (n = 3 biological replicates). *P < 0.05, **P < 0.01 vs. Cntl (unpaired two-tailed Student's t test).