Fig 1. Effects of iκB-α and IL-1β-inducible NF-κB activation.

Caco-2 cells were transfected with pNF-κB-Luc for 18 hours, then treated±IL-1β (4 ng/ml) for 4 hours. Cell lysates were assayed for luciferase activity and protein concentration as described in Methods. Data are expressed as fold induction of normalized luciferase activity ± SE. * P<0.05 (n = 6 /group).