Fig 4. Glucose effects on metformin-inhibited cell invasion.

(A & B) AGS cells were treated with metformin for 24 hours and the cell invasion was measured in the Culturex 96 well BME invasion assay kit by counting the number of cells invading underside of BME (*p ≤ 0.01, **p ≤ 0.001 and ***p ≤ 0.0001). (C) Effect of high- and low-glucose media on cell invasion analyzed by JMP and levels not connected by same letter are significantly different (p ≤ 0.05).