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. 2017 Mar 9;13(3):e1006654. doi: 10.1371/journal.pgen.1006654

Fig 6. Cuzd1(-/-) mammary glands do not undergo alveologenesis in response to hormone treatment.

Fig 6

(A) Cuzd1(-/-) mammary gland morphology in response to hormonal treatment. Cuzd1(+/+) and Cuzd1(-/-) mice (n = 5) were treated with a vehicle control (a and b) or E+P+PRL (c and d) for 3d. Whole mount analysis of no. 4 inguinal mammary glands of Cuzd1(+/+) and Cuzd1(-/-) mice after 3d of hormone treatment. Magnification 6.3x. (B) Analysis of CUZD1 expression. Mammary tissue sections obtained from Cuzd1(+/+) (a and c) and Cuzd1(-/-) (b and d) mice were subjected to IHC using an antibody specific for CUZD1. (C) Activation of STAT5. Mammary tissue sections obtained from Cuzd1(+/+) (a and c) and Cuzd1(-/-) (b and d) mice were subjected to IHC using an antibody specific for pSTAT5. (D) Analysis of EREG expression. Mammary tissue sections obtained from Cuzd1(+/+) (a) and Cuzd1(-/-) (b) mice were subjected to IHC using an antibody specific for EREG. Magnification 20x.