Fig 3. Development of bradyzoites in vitro and in vivo.

(A) There was no significant difference in bradyzoite differentiation in vitro across the parasite lines in either tachyzoite conditions (left) (P> 0.99) or bradyzoite conditions (right) (P> 0.99) (Two-way ANOVA).(B) Representative pictures of tachyzoites, partial cysts, and complete cysts produced in vitro as assessed by DBL staining. (C) Brain cyst yields in mice 1–2 months post-infection. All parasite lines produced similar numbers of tissue cysts in vivo (Kruskal-Wallis, ns).