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. 2017 Mar 17;12:2129–2142. doi: 10.2147/IJN.S126016

Figure 5.

Figure 5

In vitro targeting and cytotoxicity of TPNPs.

Notes: (A) Binding of hCTL membrane-coated PLGA nanoparticles to MKN-45 cells (PLGA, green [DiO]; hCTL membranes, red [DiI]; nucleolus, blue [DAPI]) evaluated using confocal microscopy over a short (1 h) incubation time. (B) MKN-45 viability post incubation for 24 h and (C) 48 h with free PTX, PTX-loaded PLGA nanoparticles, PTX-loaded TPNPs, and (D) empty TPNPs. (E) MKN-45 viability with 2 Gy irradiation and a combination of 2 Gy irradiation with free PTX, PTX-loaded PLGA nanoparticles, and PTX-loaded TPNPs at 5 ng/mL for 24 h. Measurements were normalized to the viability of the untreated cells (100%).

Abbreviations: TPNPs, T-lymphocyte membrane-coated PLGA nanoparticles; PTX, paclitaxel; hCTL, human cytotoxic T lymphocyte; PLGA, poly-lactic-co-glycolic acid; PLGA–PTX, PTX-loaded PLGA nanoparticles; T-PLGA–PTX, PTX-loaded PLGA nanoparticles with hCTL membrane encapsulation; FITC, fluorescein isothiocyanate.