Figure 3. Comparative AP-MS for transfected SNX5WT, SNX5Y132D, and SNX5F136D.
The Y-axis quantifies the number of peptides/charge detected by LC-MS/MS for CI-MPR, SNX5, and SNX1 that affinity purified with the indicated full-length SNX5 proteins. Mock refers to untransfected cells. The graph is normalized to SNX5WT. Whereas SNX1 is equally well represented in the affinity-purified lysates from WT and mutant SNX5, the representation of CI-MPR peptides differed significantly between SNX5WT and each of the SNX5 mutants. Data are mean +/− SD from three independent biological experiments.