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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Mol Microbiol. 2017 Jan 26;104(1):105–124. doi: 10.1111/mmi.13616

Figure 4. [SWI+] spontaneous de novo formation promoted by pre-existing [PSI+] or [PIN+].

Figure 4

(A) Representative growth phenotypes of the BY4741 strain-derived [SWI+] candidate isolates before and after 5 mM GdnHCl treatment. The [SWI+] converting frequency is summarized in panel B. +5FOA (SC-his+5-FOA); −ura (SC-his-ura); raf (raffinose). (B) As described in the Experimental Procedures, [SWI+] spontaneous conversion frequencies in the indicated strain backgrounds were summarized based on at least three independent experiments. (C) Appearance, maturation and curability of Swi1-NQ-YFP aggregation during Swi1 prionogenesis in 74D-694 strains. Upon colony purification, aggregation appeared in 5-FOA+ cells could be stabilized, morphologically remodeled, and curable. Experiments were done for the [PSI+], [PIN+] and nonprion strains, and [SWI+] converting frequencies are summarized in panel B. Shown here are representative data (upper, from non-prion strain; lower, ring/ribbon-like aggregates from [PSI+] and [PIN+] strains). Statistical analysis was performed by T test (**, P<0.01).