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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2017 Feb 2;37(4):664–674. doi: 10.1161/ATVBAHA.116.308791

Figure 2. Tamoxifen inhibits platelet activation.

Figure 2

To measure platelet activation, platelets were isolated from healthy donors, pretreated with 10–20 µM of tamoxifen or vehicle control, washed, and exposed to agonists (ADP, MCF-7 tumor cells or TRAP). (A). P-selectin surface expression, a marker of activation, was determined by flow cytometry. Representative histograms are shown, with P-selectin stained platelets (gray) overlaid onto platelets stained with isotype control antibodies (red) (B). Quantified results are shown in C. Bars indicate SEM. P<*0.01 compared to resting control unless otherwise indicated by ANOVA, n=3–6 independent replicates per treatment group.