Figure 1.
Circulating exosomal miR-155 levels increased over the course of experimental cerebral malaria (ECM) and were associated with ECM susceptibility in P. berghei ANKA (PbA) infection. (A) C57BL/6 mice were more susceptible to PbA infection than BALB/c mice. Survival of C57BL/6 mice was compared with BALB/c mice infected with 1 × 105 parasitized erythrocytes of PbA. Survival was significantly less in PbA-infected C57BL/6 than BALB/c mice (****P < 0.0001; log-rank test n = 9–10/group). (B) Exosomal miR-155 levels increased over the course of infection in ECM-susceptible mice. Median circulating exosomal miR-155 levels, measured by qRT-PCR, were significantly higher in C57BL/6 than BALB/c mice at d 5, immediately before the onset of ECM (****P < 0.0001; two-way ANOVA with Tukey’s multiple comparisons test; n = 4-5/group). Error bars represent standard deviation.