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. 2017 Mar 24;8:488. doi: 10.3389/fmicb.2017.00488

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Copyright © 2017 Ansari, Abraham, Massaro, Murphy, Smith-Carpenter and Fikrig.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Clumping and detachment of exopolymeric matrix in the presence of P1. (A) S. mutans cultures in well of 12-well plate after overnight incubation with peptide P1 or sP1 at 0.1 mg/ml. Detached chunks of floating matrix material are visible in P1-treated sample (no magnification). (B) Cells and exopolymeric matrix (same samples from A) viewed through 10× objective with phase contrast microscopy. Bar = 100 μm. Cells and matrix appear dark, clear areas show lack of biofilm attached to well. (C) Matrix clumping in wells of 24-well plate. (D) Biofilm eDNA (same samples from C) stained with fluorescent Miami Orange probe and viewed under fluorescent light with 20× objective. Bar = 100 μm.