Figure 4.

Investigation of the effect of H1C on influenza A viral replication and proliferation in A549-H1C-KO cells. (A) A549-H1C-KO cells transfected with H1C or its mutants were then infected with influenza virus (WSN, MOI = 0.01). The supernatants were collected at 12, 24, and 36 h post-infection, and the viral titers were detected using plaque assay on MDCK cells. (B) A549-H1C-KO cells were treated as described above, and the RNA was collected at 12, 24, and 36 h post-infection, and NP mRNA was detected using real-time PCR. (C) Cells were treated as described above, and western blotting analyses were conducted to detect the NP expression levels (*p < 0.05, **p < 0.01, the data were generated from three independent experiments).