Figure 5.

Analysis of the effects of H1C on cytokines and chemokines. A549 cells were transfected with small interfering RNA (siRNA), H1C or its mutants and were harvested at 24 h later. The mRNA of target genes [interferon-β (IFN-β), IL-6, IL-8, TNF-α, OASL, MX1, and CXCL10] were detected using real-time PCR, a non-target siRNA (Si-NC), and the siRNA targeted to GAPDH (Si-GAPDH) were used as a negative or positive control to detect the silencing specificity of H1C (*p < 0.05, **p < 0.01, the data were generated from three independent experiments).