Figure 7.

Effect of different ABC transporter inhibitors on the percentage of Sb released from WT (A) and Sb^III-resistant (B) L. braziliensis and WT (C) and Sb^III-resistant (D) L. guyanensis promastigotes, after 2 h of efflux. Cells were first exposed for 1 h at 25°C to 1 mM Sb^III, were washed and resuspended in HG buffer at 108 cells/mL and were incubated for 2 h at 25°C in the absence or presence of the inhibitor (8 μM verapamil, 4 mM probenecid, 10 μM prochlorperazine, 100 μM BSO, 10 mM sodium azide in resistant strains and 8 μM verapamil, 4 mM probenecid, 3.5 μM prochlorperazine, 100 μM BSO, 10 mM sodium azide in parental strains). Only when evaluating the effect of azide, that the buffer did not contain glucose. The cells were finally washed and processed to determine the cellular content of Sb. The data are shown as the percentage of Sb released for 2 h in relation to the initial cellulart Sb content. The data comes from at least three independent experiments and are shown as means ± SEM. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 for statistical comparison to Control (without inhibitor), using One-way ANOVA followed by Bonferroni multiple comparison test.