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. 2017 Mar 24;11:150. doi: 10.3389/fnins.2017.00150

Figure 2.

Figure 2

Overexpression of ASPP2 inhibits autophagy in SH-SY5Y neuroblastoma cells when treated by 50 ng/mL gp120 but promotes autophagy when treated by 200 mg/ml gp120. (A) Bands of ASPP2, Beclin1, LC3-I/LC3-II of SH-SY5Y cells which were infected with ASPP2-rAd and the control rAd for 36 h then treated by 50 and 200 ng/mL gp120, respectively. (B) Data of the LC3-II/actin. (C) Relative levels of Beclin1 normalized with beta-actin. (D) The detection of cells with GFP-LC3 positive puncta when treated by 50 and 200 ng/mL gp120, respectively. Representative images are shown (with 100× objective lens). (E) The mean levels of GFP-LC3 positive puncta cells treated with different concentrations of gp120. A total of 100 cells were counted in each group. All data were shown as the mean ± S.E.M. of three independent experiments, *p < 0.05, **p < 0.01.