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. 2017 Mar 24;11:150. doi: 10.3389/fnins.2017.00150

Figure 4.

Figure 4

The silence of ASPP2 promoted autophagy and apoptosis in SH-SY5Y cells in the presence of low dose gp120 and decreased autophagy and apoptosis in the presence of high dose gp120. (A) Bands of ASPP2, Beclin1, and LC3-I/LC3-II of SH-SY5Y cells which were transfected by ASPP2 siRNA and control siRNA then treated by 50 ng/mL gp120. (B) Relative levels of Beclin1. (C) Data of the LC3-II/actin in different groups. (D) Detection of cells with GFP-LC3 positive puncta of autophagy in different group. (E) The mean levels of cells with autophagy in different groups, a total of 100 cells were counted in each group. (F) Bands of ASPP2, Beclin1, and LC3-I/LC3-II of SH-SY5Y cells which were transfected by ASPP2 siRNA and control siRNA then treated by 200 gp120. (G) Relative levels Beclin1 in different groups when treated by 200 gp120. (H) Data of LC3-II/actin in different groups. (I) Detection of SH-SY5Y cells with GFP-LC3 positive puncta of autophagy in different groups when treated by 200 ng/mL gp120. (J) The mean levels of autophagy cells in different groups when treated by 200 ng/mL gp120. A total of 100 cells were counted in each group and data was shown as the mean ± S.E.M of three independent experiments. *P < 0.05; **P < 0.01.