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. 2017 Mar 24;7:94. doi: 10.3389/fcimb.2017.00094

Figure 6.

Figure 6

NF-κB-dependent gene expressions induced by TLR4 are increased in Prdx6KD THP-1 cells. (A) Comparison of NF-κB-dependent gene expression in control (Ctrl) vs. Prdx6KD THP-1 cells. Ctrl and Prdx6KD THP-1 cells were untreated or treated with LPS (200 ng/ml) for different times, as indicated. Total RNA was extracted, and microarray analysis was performed, as described in Methods. NF-κB-dependent gene expressions were compared in Ctrl THP-1 vs. Prdx6KD THP-1 cells. Gene expression levels are indicated with color bars (Red, up-regulated genes; green, down-regulated genes) (B) Ctrl and Prdx6KD THP-1 cells were untreated or treated with LPS (200 ng/ml) for different times, as indicated. Total RNA was extracted, and quantitative RT-PCR analysis with specific primers to IL-1β, IL-8, NFκB1, CD44, RELB, and CCL5, was performed. All error bars represent ± SEM of the mean from triplicate samples. IL-1β:#p = 0.0014 and ##p = 0.0179; IL-8: #p = 0.0015; NFκB1:#p = 0.0034 and ##p = 0.0004; CD44: #p = 0.0222 and ##p = 0.0063; RELB: #p = 0.0072 and ##p = 0.0102: CCL5; #p = 0.0078 and ##p = 0.0112.