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Differential expression patterns of several known miRNAs and novel miRNAs in different developmental phases of broccoli pollen validated by quantitative stem-loop RT-PCR. The small nuclear RNA U6 was used as an internal reference. ** indicated the significantly differential expression level with P < 0.01. UM, BCP, and TCP indicated the uninucleate microspores, binucleate pollen grains, and trinucleate pollen gains, respectively.
