Table 1.

The predicted novel miRNAs in broccoli uninucleate microspores, binucleate pollen grains, and trinucleate pollen grains.

Novel miRNAs	Sequence (5′-3′)	MFE (kcal/mol)	Novel miRNAs	Sequence (5′-3′)	MFE (kcal/mol)
bol-miR01	GGAATGTTGTTTGGCTCGAAG	−20	bol-miR29	TAATCATGTTTAGACTTAGATCA	−46.7
bol-miR02	AGATATTAGTGCGGTTCAATC	−44.5	bol-miR30	GGACGTGCTGTAGGAGTAACCC	−28.7
bol-miR03	GGCCGTGGGATGTGGATGGCA	−45.5	bol-miR31	GGAGGATGACGGAGAAGGAGCA	−60.5
bol-miR04	ATGCACTGCCTCTTCCCTGGC	−38.2	bol-miR32	TCTGGTGTAGACGTGGTATC	−48.9
bol-miR05	GTTCTTAAAGTAGATCTTCGGAA	−44.7	bol-miR33	ACAGCTCTGCTCCATCTGTGA	−85.3
bol-miR06	AATGAAATAAGAAGGCGAGGATT	−37.3	bol-miR34	TGGCTGCTGGAATTGATGGTC	−34.7
bol-miR07	TGCATCAACTGAATCGGAGCC	−73.5	bol-miR35	TCCCTTTGGATGTCGTCTTGTG	−30.1
bol-miR08	TCTCAAACCGTGATGATGGACG	−30.1	bol-miR36	GTGTTGTTTGACTTGAGATGACG	−44.1
bol-miR09	TCTGAAACTTTGTGACATTGCAG	−57.4	bol-miR37	ACTTCACATTGTGGTCGTCTGA	−37.5
bol-miR10	TAGCTAGTAGATGTTGTCGTG	−22.8	bol-miR38	CCAGATCTGGGATTGGCCAAC	−43.9
bol-miR11	CTTCGTGTAAATGATTTTCCTT	−33.3	bol-miR39	TCTCAGTGGATTTCGAATGGA	−29.6
bol-miR12	CAGCGAAGAGGATGTAGCGGAG	−36.1	bol-miR40	CAGCTGTAGAGTGCTGGAAGGA	−25.4
bol-miR13	AGAACTCATGAGAAGGCTTGGTG	−29.6	bol-miR41	CGATTGTGGAAGAAAGTGGA	−34.2
bol-miR14	GGAAGACCGGTGAAACTCATCTC	−29.3	bol-miR42	GGAGAGTCGGCGTGGCATCAAG	−71
bol-miR15	ACCCTTCTCACGCAGATCAAC	−30.7	bol-miR43	AATCGGCTTGTAGCAGTGGCA	−55.2
bol-miR16	GATTGACGACGACGAGGGAGACG	−75.2	bol-miR44	CGACAGCTTCTCGTCGGTCGATA	−45
bol-miR17	CGGAGGAAACGGGTTCTCGGG	−25.8	bol-miR45	CTAAGCAGGATCCAAAGACGTT	−28.6
bol-miR18	TGGATATGATGAAATGGCATA	−29	bol-miR46	AGGTGGTTATTGGAGTCGGTTTA	−40.7
bol-miR19	AGGCAGCGAAGTAGGACTGGCTT	−38.4	bol-miR47	CAAGTTGTAGGTTAGTTTTGGCA	−34.2
bol-miR20	GAGGTATGGGACGATGGTGG	−49.7	bol-miR48	AGGAGACTGTTTATGTAAGGCTA	−28.1
bol-miR21	GCTTGTCTCTGAGATCCCGGCG	−28.5	bol-miR49	AGGACTATAGGCGAAGCGGGGTT	−37.8
bol-miR22	TTGTGCAAGACTAAGAAGCAA	−46.7	bol-miR50	CACCCTTCTCACGCAGATCAA	−35.7
bol-miR23	TGGCTAAATCCAGATATGTCG	−43.9	bol-miR51	TCCCTTTGGATGTCGTCTTGT	−30.1
bol-miR24	GCTTCTAGTGCGATCGGGTTCGG	−49.3	bol-miR52	CGTCATTTGGATCCATCGGG	−33.7
bol-miR25	GCCATGGCGGAAGAGTTTTTATC	−36.1	bol-miR53	GTGCTTATTGACGGTCTTGT	−32.5
bol-miR26	ACTTGTTTCGTCGGTATGTCG	−51.6	bol-miR54	AGGACTTGTCTTGGATAGGTATA	−29.9
bol-miR27	GACGGTTCTTAGCTTTTCTT	−25.9	bol-miR55	GTTGGAGGAGGAGGAGGAGGA	−38.8
bol-miR28	GAGCGACTGTTTCTTCGTCGGA	−56.8