Table 3.

Relative molecular weight distribution (%) of intact RSM toasted for different times and the hydrolysates of these RSM, and soluble and insoluble protein fractions separated from these RSM

Toasting time	Intact RSM			Hydrolysates
				RSM			Soluble protein fraction			Insoluble protein fraction
	>10 kDa	10–1.5 kDa	<1.5 kDa	>10 kDa	10–1.5 kDa	<1.5 kDa	>10 kDa	10–1.5 kDa	<1.5 kDa	>10 kDa	10–1.5 kDa	<1.5 kDa
0 min	52.3	12.8	34.9	19.2	21.4	59.5	33.6	26.4	40.0	11.7	32.0	56.3
20 min	50.9	9.0	40.1	13.1	24.3	62.6	27.8	27.8	44.4	7.0	35.8	57.2
40 min	49.5	10.1	40.5	12.7	25.7	61.6	24.9	28.1	47.0	7.5	36.6	56.0
60 min	48.6	9.8	41.6	13.3	28.6	58.1	22.8	30.4	46.8	9.1	37.3	53.6
80 min	45.2	10.3	44.5	12.6	28.7	58.7	20.3	30.6	49.1	11.2	38.3	50.5
100 min	42.4	11.3	46.3	13.7	30.7	55.6	19.2	31.3	49.5	13.1	39.3	47.6
120 min	38.3	12.4	49.3	14.0	30.5	55.5	18.8	30.7	50.5	15.6	39.6	44.7
SEM	1.4	0.4	1.2	0.5	0.9	0.8	1.3	0.5	0.9	0.9	0.6	1.3
P value
Linear	<0.001	0.15	<0.001c	0.34	<0.001e	<0.001f	<0.001	0.002h	<0.001i	0.003	<0.001	<0.001
Quadratic	0.04a	0.006b	0.76	0.006d	0.13	0.32	0.005g	0.18	0.07	<0.001j	0.02k	0.002l

Abbreviations: RSM, rapeseed meal; SEM, standard error of the mean

^aIntact >10 kDa = 51.84 − 0.017 × time − 0.00,079 × time² (R ² = 0.91)

^bintact 10–1.5 kDa = 11.45 − 0.07 × time + 0.00066 × time² (R ² = 0.63)

^cIntact <1.5 kDa = 36.38 + 0.10 × time (R ² = 0.85)

^dRSM >10 kDa = 16.97 − 0.13 × time + 0.00093 × time² (R ² = 0.58)

^eRSM 10–1.5 kDa = 22.83 + 0.073 × time (R ² = 0.76)

^fRSM <1 kDa = 62.63 − 0.060 × time (R ² = 0.67)

^gSoluble >10 kDa = 32.81 − 0.23 × time + 0.00099 × time² (R ² = 0.93)

^hSoluble 10–1.5 kDa = 27.09 + 0.038 × time (R ² = 0.60)

ⁱSoluble <1 kDa = 42.68 + 0.071 × time (R ² = 0.77)

^jInsoluble >10 kDa = 10.10 − 0.093 × time + 0.0012 × time² (R ² = 0.91)

^kInsoluble 10–1.5 kDa = 32.74 + 0.11 × time − 0.00041 × time² (R ² = 0.92)

^lInsoluble <1 kDa = 57.16 − 0.014 × time − 0.00078  × time² (R ² = 0.97)