Reagent or Resource	Source	Identifier
Antibodies
anti-phospho-Histone H3 (Ser 10)	Upstate	Cat#06-570
anti-ZO1	Invitrogen	Cat#33-9100
Cy5-conjugated goat anti-mouse IgG	Jackson ImmunoResearch	Cat#115-175-146
Chemicals, Peptides, and Recombinant Proteins
DAPI	Invitrogen	Cat#D1306
Hydroxyurea	Sigma-Aldrich	Cat#H8627
Aphidicolin	Sigma-Aldrich	Cat#A0781
Dextran Alexa Fluor 647	Invitrogen	Cat#D22914
Dextran fluorescein	Invitrogen	Cat#D1821
Histone H1 Alexa Fluor 647	Invitrogen	Cat#C29926
Critical Commercial Assays
mMESSAGE mMACHINE SP6 Transcription Kit	Ambion	Cat#AM1340
Experimental Models: Organisms/Strains
Zebrafish: AB	Zebrafish International Resource Center (ZIRC)	ZFIN: ZDB-GENO-960809-7
Zebrafish: chukp20ad/p20ad (poky)	Fukazawa et al., 2010	ZFIN: ZDB-FISH-150901-16711
Zebrafish: Tg(actb1:lifeact-EGFP)	Behrndt et al., 2012	N/A
Zebrafish: Tg(actb1:myl12.1-EGFP)	Maître et al., 2012	N/A
Zebrafish: Tg(krt4:EGFP-CAAX)	Choo et al., 2006	ZFIN: ZDB-ALT-111207-5
Recombinant DNA
Plasmid: Membrane-GFP	Kimmel and Meyer., 2010	N/A
Plasmid: Membrane-RFP	Iioka et al., 2004	N/A
Plasmid: H2B-EGFP	Keller et al., 2008	N/A
Plasmid: H2A-mCherry	Arboleda-Estudillo et al., 2010	N/A
Plasmid: RhoA	Takesono et al., 2012	N/A
Plasmid: Mypt1	Jayashankar et al., 2013	N/A
Software and Algorithms
Mathematica	Wolfram Research, Inc.	http://www.wolfram.com
Fiji	NIH	https://fiji.sc
Matlab2013a	MathWorks Inc.	http://mathworks.com
Octave 4.0.0	GNU Octave	http://www.octave.org
Imaris 7.4	Bitplane Inc.	http://www.bitplane.com
Packing Analyzer	Aigouy et al., 2010	N/A