FIG 2.
Pca1-specific antibody development in immunized mice. (A) Pooled sera from five mice bled 3 weeks after completing a three-dose immunization series of 80 μg of Pca1 fusion protein (high-dose Pca1) demonstrated antigen-specific antibody as measured by ELISA to a Pca1 polypeptide. Pooled sera from five mice immunized with only 10 μg of the Pca1 fusion protein (low-dose Pca1) or 80 μg of the fusion partner only (negative control) did not demonstrate significant antibody development above PBS-immunized mice (sham). OD405nm, optical density at 405 nm. (B) Pca1-specific antibody was detected in high-dose Pca1-immunized mice approximately 6 weeks following CD4+ T cell depletion and subsequent exposure to Pneumocystis, corresponding to approximately 9 weeks after the last immunization. Values are means ± standard errors of the means (SEM) (error bars) for the groups (five animals in each group).