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Pca1 antisera recognize both mouse and human Pneumocystis cysts by IFA. Antisera from mice immunized with the Pca1 fusion protein bound not only to the surface of mouse species-specific Pneumocystis (P. murina) cysts (A) but also to the surfaces of rat-specific P. carinii (B) and the human pathogen P. jirovecii (C). No such binding was observed with fusion partner-immunized antisera (D).
