TABLE 3.
Oligonucleotide primers used in this study
| Primer | Sequence (5′–3′) |
|---|---|
| NleFF1 | AAGAATTCATGTTACCAACAAGTGGTTCT |
| NleFF2 | CCCGAATTCAATGTTACCAACAAGTGGTTC |
| NleFF3 | ATGTTACCAGCTAGTAGC |
| NleFR1 | GCGGTGGATCCCTCATCCACATTGTAA AGATCC |
| NleFR2 | GGGGGATCCTCAAGATCCTTTGTTGTAAG |
| NleFR3 | GGGGCTAGCTCATCCACATTGTAAAGATCC |
| NleFR4 | GGGGCTAGCTCAAGATCCTTTGTTGTAAG |
| NleFR5 | ATCCTCGAGCTATCCAATTTGCAAAGATC |
| FlagF | CAGGATCCATGGACTACAAAGACCATGACGGTG |
| NleB1F1 | AAGCGAAT TCAATGTTATCTTCATTAAATGTCC |
| NleB1F2 | GTGTTTACGAAGCAAAAGCTTAGTCTAAGGGGAATCTT |
| NleB1R1 | GCGGTGGATCCCTTACCATGAACTGCTGGTATAC |
| NleB1R2 | GGGGCTAGCTTACCATGAACTGCTGG |
| NleB1R3 | ATCCTCGAGTTACCATGAACTGTTGGTAT |
| NleB1(DXD)F | GCGGATGTATATATCTTGCTGCCGCCATGATTATTACCG |
| NleB1(DXD)R | CGGTAATAATCATGGCGGCAGCAAGATATATACATCCGC |
| NleFEP_1 | GGTTCCGGGAGTGCAGAGAAA |
| NleFEP_2 | GAAGCAGCTCCAGCCTACACAAAAACCCCCTTAACAAATAAATCC |
| NleFEP_3 | CTAAGGAGGATATTCATATGATTAGAGGCCTGTGAGCCTGT |
| NleFEP_4 | ATGCATCGTGCATATGGTGC |
| NleFCR_1 | GTAAAGGTGGTAACGCCGTG |
| NleFCR_2 | GAAGCAGCTCCAGCCTACACACATGCCTACTATAACCGGATAAAAA |
| NleFCR_3 | CTAAGGAGGATATTCATATGGGGCCTGGTTACCATTCTGT |
| NleFCR_4 | CACCCCCATCCTCTTAGCAG |
| S12F1 | ATCCCGGGGCGCGATCGTTGTATATTTC |
| S12R1 | AGCTTTTGCTTCGTAAACAC |
| S12R2 | GCTACTAGCTGGTAACATTAAATAGCTCCTGGTTTT |
| pKDF | TGTGTAGGCTGGAGCTGCTTC |
| pKDR | CATATGAATATCCTCCTTAG |
| CRF | AGAGATCTCATACATTCACG |
| CRR | TGGCTTATAATCTGATACGG |