FIG 4.

Expression of cytokines in brains of mice following T. gondii infection. (A) Expression of IL-1β, TNF-α, and IFN-γ in brains of wild-type BALB/c mice after injection with PBS or infection with T. gondii at 10 dpi under treatment with Mino (10 mg/kg i.p.) or injection with PBS from days 4 to 7 postinfection. Data are representative of data from two independent experiments with similar results and are presented as means ± standard deviations [n = 5 or 6 (one mouse died due to infection in the T. gondii-infected and PBS-injected group); for IL-1β, F_{(1, 19)} = 13.66 and P = 0.0015; for TNF-α, F_{(1, 19)} = 8.235 and P = 0.0098; for IFN-γ, F_{(1, 19)} = 6.325 and P = 0.0211]. (B) Expression of IL-1β and TNF-α in brains of wild-type BALB/c mice (IFN-γ^+/+) and IFN-γ^−/− mice after injection with PBS or infection with T. gondii at 10 dpi. Data from two independent experiments are summarized and presented as means ± standard deviations [n = 5 plus 5; for IL-1β, F_{(1, 36)} = 35.98 and P < 0.0001; for TNF-α, F_{(1, 36)} = 50.97 and P < 0.0001]. (C) Expression of IL-1β, TNF-α, and IFN-γ in brains of wild-type BALB/c mice after injection with PBS or infection with T. gondii at 10 dpi under treatment with 1-MT (50 mg/kg subcutaneously) or injection with the vehicle from days 4 to 7 postinfection. Data are representative of data from two independent experiments with similar results and are presented as means ± standard deviations [n = 5 or 6 (one mouse died in the T. gondii-infected and vehicle-injected group); for IL-1β, F_{(1, 19)} = 0.937 and P = 0.3452; for TNF-α, F_{(1, 19)} = 0.1289 and P = 0.7235; for IFN-γ, F_{(1, 19)} = 0.1108 and P = 0.7429]. Different letters above bars in the graphs indicate statistically significant differences among the groups by two-way ANOVA plus Tukey-Kramer post hoc analysis.