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. 2017 Mar 23;85(4):e00007-17. doi: 10.1128/IAI.00007-17

FIG 6.

FIG 6

Trp turnover to Kyn or 5-HT in mice following T. gondii infection. Trp turnover to Kyn (Kyn/Trp ratio) in plasma and brain and Trp turnover to brain serotonin (5-HT/Trp ratio) were determined. (A) Trp turnover in wild-type BALB/c mice after injection with PBS or infection with T. gondii at 10 and 30 dpi. Plasma Kyn/Trp ratios are presented as means ± standard deviations [n = 5; F(1, 16) = 8.039; P = 0.0119]. Data for brain Kyn/Trp and brain 5-HT/Trp ratios from two independent experiments are summarized and presented as means ± standard deviations [n = 4 or 5 plus 5; for the brain Kyn/Trp ratio, F(1, 32) = 274.6 and P < 0.0001; for Trp turnover, F(1, 36) = 0.01294 and P = 0.9101]. (B) Trp turnover in wild-type BALB/c mice after injection with PBS or infection with T. gondii at 10 dpi under treatment with Mino (10 mg/kg i.p.) or injection with PBS from days 4 to 7 postinfection. Data are presented as means ± standard deviations [n = 5 or 6 (one mouse died in the T. gondii-infected and PBS-injected group); for the plasma Kyn/Trp ratio, F(1, 19) = 15.91 and P = 0.0008; for the brain Kyn/Trp ratio, F(1, 19) = 12.44 and P = 0.0023; for Trp turnover, F(1, 19) = 0.2663 and P = 0.6118]. (C) Trp turnover of wild-type BALB/c mice (IFN-γ+/+) and IFN-γ−/− mice after injection with PBS or infection with T. gondii at 10 dpi. Data from two independent experiments are summarized and presented as means ± standard deviations [for the plasma Kyn/Trp ratio, n = 4 plus 4, F(1, 28) = 21.44, and P < 0.001; for the brain Kyn/Trp ratio, n = 5 plus 5, F(1, 36) = 6.016, and P = 0.0192; for the brain 5-HT/Trp ratio, n = 5 plus 5, F(1, 36) = 3.19, and P = 0.0825]. (D) Trp turnover of wild-type BALB/c mice after injection with PBS or infection with T. gondii at 10 dpi under treatment with 1-MT (50 mg/kg subcutaneously) or injection with the vehicle from days 4 to 7 postinfection. Data are presented as means ± standard deviations [n = 5 or 6 (one mouse died due to infection in the T. gondii-infected and vehicle-injected group); for the plasma Kyn/Trp ratio, F(1, 16) = 7.838 and P = 0.0129; for the brain Kyn/Trp ratio, F(1, 19) = 11.51 and P = 0.0031; for Trp turnover, F(1, 19) = 4.43 and P = 0.0489]. Different letters above bars in graphs indicate statistically significant differences among the groups by two-way ANOVA plus Tukey-Kramer post hoc analysis.