FIG 5.
Dose response and specificity of RplJ-specific T cell hybridomas. The rplJTB146–160-specific TCH clone “RplJ.03” was used to further establish the dose dependence and specificity of T cell responses to the rplJTB146–160 ribosomal peptide epitope (A) or the ribosome-enriched fraction from IKEPLUS (B). C57BL/6 BMDCs were used as APCs, and responses were quantified by an IL-2 ELISA. Control TCHs specific for MHC class II-restricted M. tuberculosis Ag85B or ovalbumin (OVA) peptides gave no detectable responses to either rplJTB146–160 or the ribosome-enriched fraction from IKEPLUS. Data are mean values and standard errors for duplicate samples and are representative of results from three independent experiments.