FIG 10.

NFI-A conditional knockout mice that had recovered from sepsis mount a normal inflammatory response to LPS challenge. A number of the NFI-A conditional knockout mice that had recovered from sepsis were challenged (i.p.) with a sublethal dose (25 μg) of the Gram-negative bacterial endotoxin lipopolysaccharide (LPS) (from Escherichia coli serotype 0111:B4; Sigma-Aldrich, St. Louis, MO). Mice were subjected to the LPS challenge 2 weeks after the end of the 4-week sepsis time course. A group of naive, control mice were also injected with LPS and served as a positive control. Blood was collected 24 h later, and plasma levels of TNF-α and IL-6 were determined by ELISA. Data are expressed as means ± SD of results from 5 mice per group and represent one of two ELISAs. cKO, conditional knockout.