TABLE 3.
Strains and plasmids used in this study
| Strain and genotype or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Y. pseudotuberculosis | ||
| wt | IP32953 serotype I | E. Carniela |
| wtL | wt 16S rRNA::luxABCDE Eryr | This study |
| tatC::cml | IP32953 with tatC insertion mutation, Cmlr | 22 |
| ΔtatC | IP32953 with tatC in-frame deletion | 23 |
| ΔtatCL | IP32953 ΔtatC 16SrRNA::luxABCDE, Eryr | This study |
| sufI::cml | IP32953 with sufI insertion mutation, Cmlr | 23 |
| ΔsufI | IP32953 with sufI in-frame deletion | This study |
| ΔsufIL | IP32953 ΔsufI 16SrRNA::luxABCDE, Eryr | This study |
| ΔcynT | IP32953 with cynT in-frame deletion | This study |
| ΔycbK | IP32953 with ycbK in-frame deletion | This study |
| ΔYPTB3260 | IP32953 with YPTB3260 in-frame deletion | This study |
| ΔYPTB1325 | IP32953 with YPTB1325 in-frame deletion | This study |
| fhuD::cml | IP32953 with fhuD insertion mutation, Cmlr | 23 |
| cueO::cml | IP32953 with cueO insertion mutation, Cmlr | 23 |
| amiC::cml | IP32953 with amiC insertion mutation, Cmlr | 23 |
| ybtP::cml | IP32953 with ybtP insertion mutation, Cmlr | 23 |
| yedY::cml | IP32953 with yedY insertion mutation, Cmlr | This study |
| efeB::cml | IP32953 with efeB insertion mutation, Cmlr | This study |
| pepP::cml | IP32953 with pepP insertion mutation, Cmlr | This study |
| nicO::cml | IP32953 with nicO insertion mutation, Cmlr | This study |
| dmsA::cml | IP32953 with dmsA insertion mutation, Cmlr | This study |
| napA::cml | IP32953 with napA insertion mutation, Cmlr | This study |
| dmsA2::cml | IP32953 with dmsA2 insertion mutation, Cmlr | This study |
| tauA::cml | IP32953 with tauA insertion mutation, Cmlr | This study |
| fdoG::cml | IP32953 with fdoG insertion mutation, Cmlr | This study |
| YPTB3074::cml | IP32953 with YPTB3074 insertion mutation, Cmlr | This study |
| urtA::cml | IP32953 with urtA insertion mutation, Cmlr | This study |
| YPTB2692::cml | IP32953 with YPTB2692 insertion mutation, Cmlr | This study |
| YPTB0523::cml | IP32953 with YPTB0523 insertion mutation, Cmlr | This study |
| ΔcynT::cml | IP32953 with cynT in-frame deletion and YPTB0523 insertion mutation, Cmlr | This study |
| ΔycbK::cml | IP32953 with ycbK in-frame deletion and YPTB0523 insertion mutation, Cmlr | This study |
| ΔYPTB3260::cml | IP32953 with YPTB3260 in-frame deletion and YPTB0523 insertion mutation, Cmlr | This study |
| ΔYPTB1325::cml | IP32953 with YPTB1325 in-frame deletion and YPTB0523 insertion mutation, Cmlr | This study |
| E. coli | ||
| S17-1 λpir | RP4-2 Tc::Mu-Km::Tn7 (λpir)c | 61 |
| TOP10 | F− mcrAΔ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 araD139Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| Plasmids | ||
| pNQ705 | Suicide integration vector, Cmlr | 62 |
| pUA1 | pNQ705 with bases 80–391 of fhuD, Cmlr | 23 |
| pUA2 | pNQ705 with bases 80–391 of cueO, Cmlr | 23 |
| pUA3 | pNQ705 with bases 125–437 of sufI, Cmlr | 23 |
| pUA4 | pNQ705 with bases 100–413 of amiC, Cmlr | 23 |
| pUA5 | pNQ705 with bases 146–315 of ybtP, Cmlr | 23 |
| pUA6 | pNQ705 with bases 118–441 of yedY, Cmlr | This study |
| pUA7 | pNQ705 with bases 25–373 of efeB, Cmlr | This study |
| pUA8 | pNQ705 with bases 20–354 of pepP, Cmlr | This study |
| pUA9 | pNQ705 with bases 28–339 of nicO, Cmlr | This study |
| pUA10 | pNQ705 with bases 133–443 of dmsA, Cmlr | This study |
| pUA11 | pNQ705 with bases 135–445 of napA, Cmlr | This study |
| pUA12 | pNQ705 with bases 124–434 of dmsA2, Cmlr | This study |
| pUA13 | pNQ705 with bases 27–333 of tauA, Cmlr | This study |
| pUA14 | pNQ705 with bases 147–457 of fdoG, Cmlr | This study |
| pUA15 | pNQ705 with bases 78–456 of YPTB3074, Cmlr | This study |
| pUA16 | pNQ705 with bases 36–483 of urtA, Cmlr | This study |
| pUA17 | pNQ705 with bases 19–272 of YPTB2692, Cmlr | This study |
| pUA066 | pNQ705 with bases 71–310 of YPTB0523, Cmlr | 57 |
| pDM4 | Suicide vector, Cmlr | 63 |
| pDM4cynT | ΔcynT in pDM4, Cmlr | This study |
| pDM4tatC | ΔtatC in pDM4, Cmlr | 23 |
| pDM4sufI | ΔsufI in pDM4, Cmlr | This study |
| pDM4ycbK | ΔycbK in pDM4, Cmlr | This study |
| pDM4YBTP3260 | ΔYBTP3260 in pDM4, Cmlr | This study |
| pDM4YBTP1325 | ΔYBTP1325 in pDM4, Cmlr | This study |
| pBAD24 | Expression vector, araB, Ampr | 64 |
| pBAD24-sufIss-mCherry | pBAD24 with sufIss::mCherry, Ampr | This study |
| pCR2.1-TOPO | Cloning vector, Ampr | Invitrogen |
| pCR2.1-TOPO-ybtP-FLAG | PCR2.1-TOPO with ybtP::FLAG | This study |
| pMMB207 | Expression vector, tac, Cmlr | 65 |
| pMMB207-yptP-FLAG | pMMB207 with ybtP::FLAG, Cmlr | This study |
a
Institute Pasteur, Paris, France.