Table 2. ANI values for paired comparisons between F. prausnitzii strains whose genome has been fully sequenced.
|
ANIba
values |
ANImb
values |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| F. prausnitzii isolate | KLE1255 (ND) | A2-165 (II) | L2/6(II) | SL3/3(I) | F. prausnitzii isolate | KLE1255 (ND) | A2-165 (II) | L2/6(II) | SL3/3(I) |
| M21/2 (I) | 85.26 | 83.29 | 82.11 | 96.70c | M21/2(I) | 89.02 | 88.52 | 88.07 | 97.34c |
| KLE1255 (ND) | – | 82.79 | 82.46 | 84.70 | KLE1255 (ND) | – | 88.31 | 88.65 | 88.82 |
| A2-165 (II) | 82.77 | – | 82.60 | 82.74 | A2-165(II) | 88.31 | – | 88.23 | 88.28 |
| L2/6(II) | 82.33 | 82.87 | – | 81.61 | L2/6(II) | 88.65 | 88.23 | – | 87.99 |
Abbreviations: ANI, Average nucleotide identity; DDH, DNA-DNA hybridization; ND, not determined. Phylogroup for each strain is indicated in brackets.
a
ANIb, ANI based on BLAST searches of 1 kb genome fragments against a target genome.
b
ANIm, ANI based on the MUMmer algorithm that does not require the artificial generation of 1 kb fragments.
c
It has been shown that ANI values higher than 94% embraces organisms sharing DDH values higher than 70% which are considered to be genomospecies. ANIb has better application for distant genomes comparison, whereas both algorithms give nearly identical values in the high identity range (80–100%). Values corresponding to the same genomospecies are indicated in boldface.