Figure 4.

PR-RNA Droplets Are Dynamic Liquid Compartments

(A) Fluorescently tagged PR₂₀ and polyU RNA colocalize in phase separated droplets. Scale represents 10 μm.

(B) Spontaneous fusion behavior of PR₂₀-polyU RNA droplets over time, as expressed by changes in hydrodynamic radius over time as measured by DLS. Successive additions of polyU RNA indicated by red arrows. Final RNA concentration (green). The radius of polyU is 22nm (green line).

(C) Cryo-TEM of two PR₂₀-polyU droplets (green) wetting carbon support (brown). No obvious structures can be observed within the droplets.

(D) Full droplet bleach of PR₂₀ fluorescence indicates exchange between droplet and solution. Scale represents 5 μm.

(E) Within-droplet bleach on old versus young droplets indicates that PR₂₀-polyU droplets do not mature or age. Mean ± SD, n = 12 droplets.

See also Figure S2.