Figure 5.

PR₃₀ Initiates Phase Separation of RNA Granule Components and Metastable Proteins In Vitro

(A) Addition of PR₃₀ to cleared cell lysate induces LLPS with the formation of an insoluble fraction. Background from pre-cleared lysate not treated with PR was effectively zero. Weak interactions can be stabilized by mild PFA crosslinking.

(B) The PR₃₀ interactome (n = 874) is enriched for GO biological processes centered on RNA and protein metabolism. Benjamini-Hochberg.

(C and D) The PR₃₀ interactome significantly overlaps with cellular liquid compartments (Andersen et al., 2005, Jain et al., 2016) (C) and metastable proteins (Kato et al., 2012, Olzscha et al., 2011) (D). Binomial test.

(E and F) The PR₃₀ interactome is enriched for RNA binding domains (E) and arginine-rich motifs (Mitrea et al., 2016) (F). Binomial test.

(G and H) The PR₃₀ interactome is enriched for disordered (G) and supersaturated proteins (Ciryam et al., 2013) (H). Boxplots, whiskers indicate range. Mann-Whitney.

See also Figures S3–S5 and Table S1.