Figure 6.

PR₁₀₀ Expression Alters Stress Granule Dynamics in Cells

(A) Expression of PR₁₀₀ in HeLa cells induces cytoplasmic SGs assembly positive for PR.

(B) PR₁₀₀ is more efficient at inducing SGs than PA₁₀₀.

(C) Arsenite and puromycin increase number of SGs in PR₁₀₀ transfected cells, whereas cyclohexamide decreases them.

(D) Non-phosphorylatable form (AA) of eIF2α prevents PR₁₀₀-induced SG assembly in MEFs.

(E) G3BP1/2 knockout prevents PR₁₀₀ induced SG assembly in U2OS cells.

(F) PR₁₀₀-induced SGs have a mildly reduced FRAP recovery for G3BP1 compared to arsenite-induced ones. Repeated-measures ANOVA. Mean ± SEM, n = 17 SGs.

(G) SG enrichment of several RNA binding proteins is altered in PR₁₀₀-induced SGs compared to arsenite-induced ones. Boxplots, whiskers indicate range. Mann-Whitney. n > 60 SGs, from three independent experiments.

Fischer’s exact test. n ≥ 100 cells, from three independent experiments (A–E).

See also Figure S6.