REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-FLAG, mouse	Sigma-Aldrich	F3165; RRID: AB_259529
Anti-FLAG, rabbit	Cell Signaling	#2368S
Anti-G3BP1, mouse	Abcam	Ab56574; RRID: AB_941699
Anti-DDX6, rabbit	Abcam	Ab40684; RRID: AB_941268
Anti-YB1, rabbit	Abcam	Ab76149; RRID: AB_2219276
Anti-TDP-43, rabbit	Proteintech	12892-1-AP; RRID: AB_2200505
Anti-Ataxin-2, mouse	BD Biosciences	611378; RRID: AB_398900
Anti-TIA1, goat	Santa Cruz	sc-1751; RRID: AB_2201433
Bacterial and Virus Strains
E. coli, BL21 Star (DE3)	Thermo Scientific	C601003
Chemicals, Peptides, and Recombinant Proteins
PR20	This paper	N/A
PR30	This paper	N/A
GR30	This paper	N/A
GP30	This paper	N/A
FUS RGG	This paper	N/A
hnRNPA1 RGG	This paper	N/A
FMRP RGG	This paper	N/A
FUS LC	Dr. Nicolas Fawzi	Burke et al., 2015
hnRNPA2 LC	Dr. Nicolas Fawzi	Burke et al., 2015
1,6-Hexanediol	Sigma-Aldrich	240117
polyU RNA	Sigma-Aldrich	P9528
poly-tyrosine	Sigma-Aldrich	P1800
PEG300	Sigma-Aldrich	81160
ANS	Sigma-Aldrich	A1028
cOmplete protease inhibitor cocktail	Sigma-Aldrich	11697498001
Arsenite	Sigma-Aldrich	S7400
Lipofectamine 3000	Thermo Scientific	L3000015
NucBlue	Thermo Scientific	R37605
Prolong Gold Antifade	Thermo Scientific	P36934
Critical Commercial Assays
Alexa Fluor 488 Protein Labeling Kit	Thermo Scientific	A10235
Alexa Fluor 568 Protein Labeling Kit	Thermo Scientific	A10238
Deposited Data
Mas spec data	This paper	PRIDE: PXD005509
Experimental Models: Cell Lines
HeLa	ATCC	CCL-2
MEF eIF2a SS	Dr. Nancy Kedersha	Emara et al., 2012
MEF eIF2a SS	Dr. Nancy Kedersha	Emara et al., 2012
U2OS	Dr. Nancy Kedersha	Kedersha et al., 2016
U2OS G3BP1/2 KO	Dr. Nancy Kedersha	Kedersha et al., 2016
U2OS G3BP1-GFP	Dr. Paul Taylor	Figley et al., 2014
Oligonucleotides
polyU30-Alexa 488	This paper	N/A
Recombinant DNA
Plasmid: PA100-FLAG	Dr. Daisuke Ito	Yamakawa et al., 2015
Plasmid: PR100-FLAG	Dr. Daisuke Ito	Yamakawa et al., 2015
Plasmid: GR100-FLAG	Dr. Daisuke Ito	Yamakawa et al., 2015
Plasmid: PR100-FLAG-mCherry	This study	N/A