Figure 5.

Function of the Elk-1 Transcriptional Activation Domain in Signal-Induced Histone Modifications at Egr1

(A) Quantitative ChIP at Egr1 with the indicated antibodies. Dashed lines, unstimulated cells; solid lines, TPA-stimulated cells; red, wild-type MEFs; black, TKO MEFs. Histone ChIP signals are normalized to total H3 (see Figure S5C).

(B) Quantitative ChIP analysis of histone modification and transcriptional machinery recruitment at Egr1 in TKO MEFs reconstituted with wild-type Elk-1 (red), Elk-1^FW (blue), Elk-1^nonA (purple), or with the empty pMY vector control (black).

(C) Signal-induced changes in H3S10ph and H3K9acS10ph at the 5′ flanking and transcribed sequences of Egr1, Egr2, Fos, and Ier2 in TKO MEFs reconstituted as in (B). Data are means ± SEM, n = 3. ^∗p < 0.05 by t test compared with TKO MEFs with empty vector.

See also Figures S5D and S5E.