Figure 5. Transcriptomes and skin histology for Tw post infection from Bsal or Bd.

(a) Skin of Tw at 10 days after exposure to Bsal (left) or Bd (right). Bsal thalli are abundantly present across the entire thickness of the epidermis (}) resulting in extensive loss of keratinocytes, whereas Bd thalli are associated with the superficial epidermal layers and hyperkeratotic foci (*). For both infections, histological evidence of an inflammatory response is lacking (Hematoxylin and eosin stain, × 400). (b) MA plots (showing Log₂ fold change in the trimmed mean of M-values (TMM) normalized Fragments Per Kilobase of transcript per Million mapped reads (FPKM) vs average Log₂ counts per million) of Tw transcripts during Bsal infection (left) and Bd infection (right) compared with non-infected. Significant differential expression is highlighted in blue (Bsal) and red (Bd), where FDR P value<0.001 and >fourfold change of TMM normalized FPKM. (c) Multiple classes of immune genes (x-axis) were found differentially expressed during Bd infection, while few were found during Bsal infection. The y-axis shows the number of genes either upregulated (positive count) or downregulated during infection (negative count).