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. 2017 Mar 20;8:14758. doi: 10.1038/ncomms14758

Figure 2. Genetic ablation of Chd7 in GNPs leads to cerebellar hypoplasia and Purkinje cell ectopia.

Figure 2

(a) Nissl staining of E15.5, P0, P7 and adult cerebella including vermis and hemisphere from [Chd7f/f] and [Atoh1-Cre::Chd7f/f] mice. Left side in each panel is the anterior part of cerebella. Scale bars, 100 μm. (b) Co-immunostaining of Calbindin (red), a marker for Purkinje cells (PCs) in P0, P3, P7 and adult WT [Chd7f/f] and Chd7 mutant [Atoh1-Cre::Chd7f/f] cerebella. Cellular nuclei are counterstained with DAPI (blue). Arrowheads indicate clusters of mislocalized PCs. Scale bars, 200 μm. Quantification of the percentage of mislocalized PCs among all PCs in anterior lobule I-V is shown in the left panel. Cerebellar sections from three independent P3 and six P7 Chd7 mutant mice were counted. (c) Immunostaining of Calbindin in cerebella of P7 and adult WT [Chd7f/f] and Chd7 mutant [Ptf1a-Cre::Chd7f/f] mice. Sections were counterstained with haematoxylin. Scale bars, 100 μm.