Skip to main content
. 2017 Mar 21;8:14772. doi: 10.1038/ncomms14772

Figure 3. Kinesin-1 preferably walks on acetylated microtubules that are highly curved and undergo bending deformations.

Figure 3

(a) Example of two trajectories with the same radius of curvature R, but different average angle between the directions of consecutive displacements. (b) Distribution of local curvatures of directed motion segments in trajectories of QDs coupled to different kinesins. (n=4,247, 8,922, 7,965, 6,041 for Kinesin-1,2,3,4). (c) Characteristic decay values from exponential fits to distributions in b. Error bars represent s.e. of fitting. (d) Average directional persistence (cosine between consecutive displacements) as a function of displacement, assuming constant average speed of kinesins (n is the same as in Fig. 2h, see also Supplementary Fig. 3a). Error bars represent s.e.m. (e) COS-7 cell expressing KIF5B-GFP (green) stained for tyrosinated (blue) and acetylated (red) tubulin. Scale bar, 10 μm. (f,g) Stills from a time lapse recording of a COS-7 cell expressing KIF5B-GFP (f) or KIF21B-GFP (g) (green) and Tubulin-TagRFP (red). Shapes of microtubules highlighted by white arrows are traced over time (s) and color-coded as indicated. Scale bars, 2 μm.