Table 2. Kinetic constants of P20-T20 hybridization measured by G-FET sensors.
Channel | 1 | 2 | 3 | 4 | 5 | 6 |
---|---|---|---|---|---|---|
ka (× 105 M−1 s−1) | 2.61 (0.11) | 2.68 (0.12) | 2.36 (0.13) | 2.73 (0.09) | 2.38 (0.20) | 2.70 (0.18) |
kd (× 10−4 s−1) | 1.08 (0.07) | 1.13 (0.04) | 1.02 (0.06) | 1.23 (0.10) | 1.10 (0.08) | 1.15 (0.07) |
KA (× 109 M−1)* | 2.35 | 2.37 | 2.31 | 2.22 | 2.16 | 2.42 |
KA (× 109 M−1)† | 2.37 | 2.30 | 2.26 | 2.23 | 2.11 | 2.39 |
*Calculated by KA=ka/kd.
†From fitting the concentration-dependent steady state.