Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 24;7:45300. doi: 10.1038/srep45300

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Total RNA was extracted from cultured prostate cancer cell lines and real time qPCR performed. Levels of all mRNAs were normalized to those of RPL19 mRNA. (b) Whole cell lysate protein (40 μg) of various prostate cancer cell lines was resolved by SDS/PAGE, and immunoblotted for the respective proteins indicated, with GAPDH intensity used as a loading control. (c) LNCaP cells were incubated for 1 day in the absence or presence of 10 nM R1881, in 1% CT-FBS medium, to optimize androgen effect(s). FASN, fatty acid synthase; PSA, prostate-specific antigen; ACCα, acetyl CoA carboxylase alpha; ACLY, acetyl-CoA lyase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; RPL19, ribosomal protein L19.